南方醫科大學張其威組在人55型腺病毒的比較基因組學研究取得進展

近日，《Frontiers in Microbiology》在線發表了南方醫科大學張其威教授課題組、中日友好醫院曹彬教授合作的研究結果：《Comparative Genomic Analysis of Re-emergent Human Adenovirus Type 55 Pathogens Associated With Adult Severe Community-Acquired Pneumonia Reveals Conserved Genomes and Capsid Proteins》。

人55型腺病毒（HAdV-B55）是近年來鑒定的一種引起急性呼吸道疾病的病原體，它屬於腺病毒B種，基因組是由引起腎臟疾病的人11型腺病毒（HAdV-B11）和引起呼吸道疾病的人14型腺病毒（HAdV-B14）重組而來（HAdV-B14的具體起源研究見先前報道：https://mp.weixin.qq.com/s/mmFqCD8VxFhh2No7IEwq6g）。自2006年HAdV-B55首次在中國某中學引起暴發以來，一直沒有進一步的相關報道，直到2011年，在中國北京暴發了成人重症社區獲得性肺炎(CAP)。隨後，HAdV-B55腺病毒在成人以及軍隊新兵中多次引起肺炎等呼吸道感染大暴發。本研究利用生物信息學方法分析闡述了再現的引起這次暴發的HAdV-B55流行株。首先，重組和蛋白序列分析再次證實了本次分離的人55型腺病毒（BJ01株）是含有HAdV-B11衣殼六鄰體基因的重組病毒。

FIGURE 1. Transcriptional map and genome organization of HAdV-B55 strain BJ01.

FIGURE 4. Schematic representation of HAdV-B55 protein VI.

三個衣殼蛋白（六鄰體、五鄰體基部、纖維蛋白）基因的進化均為負選擇，其非同義突變和同義突變的比率均很低(

Abstract

Human adenovirus type 55 (HAdV-B55) is a recently identified acute respiratory disease (ARD) pathogen in HAdV species B with a recombinant genome between renal HAdV-B11 and respiratory HAdV-B14. Since HAdV-B55 first appeared in China school in 2006, no more ARD cases associated with it had been reported until 2011, when there was an outbreak of adult severe community-acquired pneumonia (CAP) in Beijing, China. Reported here is the bioinformatics analysis of the re-emergent HAdV-B55 responsible for this outbreak. Recombination and protein sequence analysis re-confirmed that this isolate (BJ01) was a recombinant virus with the capsid hexon gene from HAdV-B11. The selection pressures for the three capsid proteins, i.e., hexon, penton base, and fiber genes, were all negative, along with very low non-synonymous (dN) and synonymous (dS) substitutions/site (

本期編輯：Tony

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