文獻：JXB揭示彈狀病毒P蛋白形成在肌動蛋白/ER網路上販運的移動內含物，用於病毒RNA合成

近日，中國農大王獻兵團隊在JXB發表的研究論文《A cytorhabdovirus phosphoprotein forms mobile inclusions trafficked on the actin/ER network for viral RNA synthesis》。

植物病毒通常劫持宿主細胞骨架以進行複製和運動。彈狀病毒是包裹的，負鏈RNA病毒，感染脊椎動物，無脊椎動物和植物，但植物彈狀病毒蛋白的細胞內運輸機制在很大程度上是未知的。

在本文中，作者使用大麥黃條紋花葉病毒（BYSMV），作為模型研究肌動蛋白細胞骨架對小複製子（MR）系統中病毒細胞內運動和病毒RNA合成的影響。

BYSMV P蛋白形成移動包涵體，其沿肌動蛋白/內質網網路被販運，並將N和L蛋白質募集到病毒體樣結構中。缺失分析顯示BYSMV P的N末端區域（aa 43-55）和剩餘區域（aa 56-295）分別對於遷移和夾雜物的形成是必需的。

肌球蛋白XI-K尾部的過表達完全消除了P body的運輸活性，並伴隨著病毒MR RNA合成的顯著減少。

這些結果表明BYSMV P蛋白有助於沿肌球蛋白XI-K驅動的ER /肌動蛋白網路形成和運輸病毒樣結構。因此，彈狀病毒P蛋白似乎是用於有效募集病毒蛋白的動態中樞蛋白，從而促進病毒RNA合成。

As obligate parasites, plant viruses usually hijack host cytoskeletons for replication and movement. Rhabdoviruses are enveloped, negative-stranded RNA viruses that infect vertebrates, invertebrates, and plants, but the mechanisms of intracellular trafficking of plant rhabdovirus proteins are largely unknown. Here, we used Barley yellow striate mosaic virus (BYSMV), a plant cytorhabdovirus, as a model to investigate the effects of the actin cytoskeleton on viral intracellular movement and viral RNA synthesis in a mini-replicon (MR) system. The BYSMV P protein forms mobile inclusion bodies that are trafficked along the actin/endoplasmic reticulum network, and recruit the N and L proteins into viroplasm-like structures. Deletion analysis showed that the N terminal region (aa 43–55) and the remaining region (aa 56–295) of BYSMV P are essential for the mobility and formation of inclusions, respectively. Overexpression of myosin XI-K tails completely abolishes the trafficking activity of P bodies, and is accompanied by a significant reduction of viral MR RNA synthesis. These results suggest that BYSMV P contributes to the formation and trafficking of viroplasm-like structures along the ER/actin network driven by myosin XI-K. Thus, rhabdovirus P appears to be a dynamic hub protein for efficient recruitment of viral proteins, thereby promoting viral RNA synthesis.

來源：植物病毒與抗逆研究

本期編輯：蘇復

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